FHIT (Fragile HistidineTriad)

May be stored at 4 °C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 °C. Aliquots are stable for at least 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recognizes human FHIT at ~17kD. Reactivity has been con rmed by Western Blot using lysates from rat brain, colon, spleen, stomach, kidney, lung, small intestine and testes as well as 293T cells and COS cells transfected with a pCMV-FHIT-DYKDDDDK construct. Species Crossreactivity: Rat. Does not react with mouse.