Hsp27, phosphorylated (Ser82)

May be stored at 4 °C for short-term only. For long-term storage, store at -20 °C. Aliquots are stable for at least 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Detects an ~27kD protein, corresponding to the apparent molecular mass of phosphorylated Hsp27 (Ser82) on SDS-PAGE immunoblots. Species crossreactivity: human, bovine, canine, chicken, guinea pig and sheep. This antibody is specific for phosphorylated Hsp27 (Ser82) and does not exhibit reactivity with non-phosphorylated Hsp27. Specificity for phospho-Hsp27 was demonstrated in peptide competition studies with phosphorylated and non-phosphorylated peptides

[HSP27; Heat shock protein beta-1; heat shock protein beta-1; HSP 27; OTTHUMP00000161096; 28 kDa heat shock protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27; estrogen-regulated 24 kDa protein; heat shock 27kDa protein 1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27]