Threonine-X-Arginine (Thr-X-Arg), phosphorylated

May be stored at 4 °C for short-term only. For long-term storage, store at -20 °C. Aliquots are stable for at least 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Detects endogenous levels of proteins containing the phospho-Thr-X-Arg motif. Detects phosphorylated Thr followed by Arg or Lys at the +2 position, though its reactivity is lower for the Lys compared to Arg at the +2 position. Does not crossreact with nonphospho-Thr or phospho-Ser in the same motif. It recognizes phospho-Thr in the FFT*R motif in PKCbeta II but does not recognize phospho-Thr in other motifs that lack Lys or Arg at +2. Recognizes all species.