Feline Leukemia Virus, p15E (FeLV)

May be stored at 4 °C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 °C. Aliquots are stable for at least 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recognizes feline leukemina virus envelope protein p15e and is specific for an epitope towards the N-terminal domain. In Western blotting, under reducing conditions, bands are observed at 20 kDa and 15 kDa representing the intact FeLV p15e and a truncated p15e sheared from the cell surface.