RLA, DQ

May be stored at 4 °C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 °C or colder. Aliquots are stable for at least 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recognizes rabbit RLA-DQ alpha chain (Mr ~35kD). Reacts with RLA-DQ-transfected cells, but not with RLA-DR-transfected cells. In normal adult rabbits, the antibody reacts with 30-60% of peripheral blood leukocytes. It has also been reported that the antibody stains: lymphocytes and macrophages in the spleen; lymphocytes in the thymic medulla and some cells in the cortex; Kupffer cells in the liver; and synovial lining cells (i.e., infiltrating macrophages) in inflamed synovium.