Nuclei and Chromosomes, Histone specific

May be stored at 4 °C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 °C. Aliquots are stable for at least 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Histone specific; stains nuclei and chromosomes of cells of all species including animals and plants. Reacts preferentially with Histone H1 by ELISA and immunoblot. Produces a speckled nuclear staining pattern in all tissues studies. Stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Nuclear staining in interphase cells is intense and diffuse.