ATPase, p97

May be stored at 4 °C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 °C or colder. Aliquots are stable for at least 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Reactivity with Species and All vertebrate cells and tissues tested so far. Cultured Cell Lines e.g. Xenopus laevis; rabbit, rat, human; Does not crossreact with the corresponding protein in budding yeast (Cdc48p). Reacts with p97 subunit of 15S Mg2+-ATPase.