Serotonin Rat Monoclonal Antibody (YC5/45)

Lyophilized antibody can be kept at 4 °C for up to 3 months and should be kept at-20 °C for long-term storage.<br>To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 °C) or for long-term storage (-80 °C).<br>For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Formaldehyde conjugate of serotonin. Without paraformaldehyde treatment, it cross-reacts in liquid phase with dopamine and tryptamine in haemagglutination tests. The antibody reacts with serotonin in neurons and recognises serotonergic sites in fixed tissue sections. It does not react with catecholamine-containing neurons in formaldehyde fixed preparations. By immunocytochemistry no cross-reactivity with dopamine and noradrenaline (6).