Cas9-IN-1

Cas9-IN-1 is a potent small-molecule inhibitor of the CRISPR-associated protein Cas9, exhibiting an IC50 of 7.02 μM. It functions by binding to apo-Cas9 and preventing its assembly with guide RNA. This inhibitor is a valuable research tool for controlling CRISPR-Cas9 activity in both in vitro biochemical assays and cellular studies.