Goat anti Rat IgE (Fc specific)

Handling:The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4 °C; prolonged storage at or below -20 °C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C . Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.<br>Packing: Vial with 10 mg lyophilized IgG fraction.<br>Storage/Shelf life: Lyophilized at +4 °C/at least 10 years<br>reconstituted at or below -20 °C/3-5 years<br>reconstituted at +4 °C/7 days

The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of IgE, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen. It does not react with rat IgG, including all subclasses, 19A, IgM and IgGlFab or any non-Ig protein in rat serum.