Mouse anti CD14, conjugated to FITC

Monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8 °C (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.

The CD14 mAb (clone MEM18) recognizes surface CD14 on human monocytes and macrophages as well as on neutrophils.<br><br>The sensitivity of MEM18 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb<br>dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI<br>(mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.

[CD14; Monocyte differentiation antigen CD14; monocyte differentiation antigen CD14; CD14 antigen; myeloid cell-specific leucine-rich glycoprotein; CD14 molecule<; Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14]