QuicKey Pro Mouse IgG2b (Immunoglobulin G2b) ELISA Kit

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG2b. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG2b are added to the micro ELISA plate wells. Mouse IgG2b in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IgG2b.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse IgG2b. The concentration of Mouse IgG2b in the samples is then determined by comparing the OD of the samples to the standard curve.