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Recombinant Human Herpesvirus Entry Mediator A Fc Chimera/TNFRSF14

Product Specifications

Gene ID

8764

UniProt

Q92956

Target

LPSCKEDEYP VGSECCPKCS PGYRVKEACG ELTGTVCEPC PPGTYIAHLN GLSKCLQCQM CDPAMGLRAS RNCSRTENAV CGCSPGHFCI VQDGDHCAAC RAYATSSPGQ RVQKGGTESQ DTLCQNCPPG TFSPNGTLEE CQHQTKCSWL VTKAGAGTSS SHWVEPKSSD KTHTCPPCPA PEFEGAPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPTP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK

Purification

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin

Less than 0.1 EU/ug of rHuHVEM-Fc/TNFRSF14 as determined by LAL method.

Bioactivity

Fully biologically active when compared to standard. The biologically active as determined by its ability to inhibit TNF-beta -mediated cytotoxicity using murine L929 cells.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculated Molecular Weight

Approximately 58 kDa, a glycosylated polypeptide chain containing 396 amino acids. But it migrates with an apparent molecular mass of 70 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4, with 3 % Trehalose.

Host or Source

Pichia. Pastoris.

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