Anti-C5aR [32-G1]

The 32-G1 antibody recognizes C5aR which has its serine 334 phosphorylated. The mAb 32-G1 reacted specifically with a C5aR peptide that contained phosphoserine 334 and also reacted with C5aRs that were isolated from C5a-stimulated, transfected RBL-C5aR cells. It did not Recognise a receptor mutant (C5aR-S-) with alanine replacement of all six C-terminal serine phosphorylation sites (Pollok-Kopp et al., 2007). Antibody 32-G1 specifically reacts with C5aR phosphoserine 334, independent of the phosphorylation state of the neighboring serine residues (Pollok-Kopp et al., 2007). Studies with Bt2cAMP-treated U937 cells show that 32-G1 specifically reacts with ligand-activated C5aR at endogenous receptor expression levels but only weakly recognizes C5aR isolated from unstimulated cells (Pollok-Kopp et al., 2007).

[C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin chemotactic receptor; C5a-R; C5a ligand; C5a anaphylatoxin receptor; complement component 5 receptor 1; complement component 5a receptor 1]