Anti-Hu CD85k Purified
CD85k is a type I transmembrane glycoprotein of ILT/LIR family, and contains three ITIM motives, which recruit protein tyrosine phosphatases SHP-1 and SHP-2, resulting in supression of signal transduction. It is expressed by monocytes, macrophages, and dendritic cells. Antigen presenting cells expressing CD85k and CD85d appear to be crucial to the generation of CD8+ suppressor T cells and CD4+ regulatory T cells.
Product Specifications
Certification
RUO
Reactivity
Human
Immunogen
Human CD85k
Target Antigen
CD85k
Clone
ZM4.1
Applications
FC (QC tested)
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Waschbisch A, Sanderson N, Krumbholz M, Vlad G, Theil D, Schwab S, Mäurer M, Derfuss T: Interferon beta and vitamin D synergize to induce immunoregulatory receptors on peripheral blood monocytes of multiple sclerosis patients. PLoS One 2014 Dec 31;9 (12) :e115488. , URL: https://pubmed.ncbi.nlm.nih.gov/25551576/, *Michalski J, Deinzer A, Stich L, Zinser E, Steinkasserer A, Knippertz I: Quercetin induces an immunoregulatory phenotype in maturing human dendritic cells. Immunobiology 2020 Jul;225 (4) :151929. , URL: https://pubmed.ncbi.nlm.nih.gov/32115260/, *Littringer K, Moresi C, Rakebrandt N, Zhou X, Schorer M, Dolowschiak T, Kirchner F, Rost F, Keller CW, McHugh D, Leibund G, Landmann S, Robinson MD, Joller N: Common features of regulatory T cell specialization during Th1 responses. Front Immunol. 2018 Jun 13;9:1344. , URL: https://pubmed.ncbi.nlm.nih.gov/29951069/, *Inui M, Sugahara-Tobinai A, Fujii H, Itoh-Nakadai A, Fukuyama H, Kurosaki T, Ishii T, Harigae H, Takai T: Tolerogenic immunoreceptor ILT3/LILRB4 paradoxically marks pathogenic auto-antibody-producing plasmablasts and plasma cells in non-treated SLE . Int Immunol. 2016 Dec;28 (12) :597-604. , URL: https://pubmed.ncbi.nlm.nih.gov/27742834/, *Chen HM, van der Touw W, Wang YS, Kang K, Mai S, Zhang J, Alsina-Beauchamp D, Duty JA, Mungamuri SK, Zhang B, Moran T, Flavell R, Aaronson S, Hu HM, Arase H, Ramanathan S, Flores R, Pan PY, Chen SH: Blocking immunoinhibitory receptor LILRB2 reprograms tumor-associated myeloid cells and promotes antitumor immunity. J Clin Invest. 2018 Dec 3;128 (12) :5647-5662. , URL: https://pubmed.ncbi.nlm.nih.gov/30352428/
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The mouse monoclonal antibody ZM4.1 recognizes an extracellular epitope of human CD85k, a transmembrane glycoprotein expressed by monocytes, macrophages, and dendritic cells.
Applications Notes
Flow cytometry: Recommended dilution: 3-6 μg/ml.
Isotype
Mouse IgG1 kappa
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