Actin (Tyr-53), Phospho-Specific

This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of pervanadate treated human C2C12 and SYF cSrc transformed cells, but not in control cells. In addition, this antibody either detects actin cleavage products at 36 and 20 kDa, or cross-reacts with unidentified proteins at these molecular weights in C2C12 cells.<br><br>All molecular weights (MW) are confirmed by comparison to MW standards and to western blot mobilites of known proteins with similar MW. "Native" western blots utilizes non-reducing sample buffer (no mercaptoethanol or SDS), normal SDS-PAGE gel electrophoresis, and no methanol in transfer buffers.