Phosphodiesterase 4C (PDE4C) ACTOne Stable Cell (CHO cell)

Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130 °C, preferably in liquid nitrogen vapor, until ready for use.<br><br>Low Survival Rate of Cells After Thawing: Cell vials could have thawed accidentally. Store cell vials in liquid nitrogen immediately after receiving and keep frozen at all time.<br>Leaving the vial at 37 °C for too long during thawing will lower the survival rate. Place the vial at 37 °C until cells are just thawed.<br>Handle the cells gently. Don't tap the vial or pipette the cells too many times before plating the cells.<br>Replace the medium four hours after thawing or when the cells have settled to remove DMSO.<br><br>Slow Growth Rate of Cells: Do not split cells before they have completely recovered from thawing and reach at least 50% confluence.<br>Do not dilute cells excessively while splitting.<br>Spit cells before they reach 80 - 90% confluence.<br>Use Trypsin-EDTA solution to dissociate cells<br>Cells may not be able to recover to an optimal stage if trypsin- free dissociation buffer is used.<br><br><b>Dry Ice Shipment: </b>Extra charge fee may add to your shipping cost as dry ice is required to ship this product.<br><br><b>Shipping Note: </b>Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.

[cAMP-specific 3',5'-cyclic phosphodiesterase 4C isoform 1; cAMP-specific 3',5'-cyclic phosphodiesterase 4C; cAMP-specific 3',5'-cyclic phosphodiesterase 4C; phosphodiesterase 4C; DPDE1; PDE21]