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Goat anti-Cathepsin B Antibody

Goat polyclonal antibody to CTSB

Product Specifications

Product Name Alternative

Anti-CTSB antibody, anti-cathepsin B antibody, anti-APPS antibody, anti-CPSB antibody, anti-APP secretase antibody, anti-amyloid precursor protein secretase antibody, anti-cathepsin B1 antibody, anti-cysteine protease antibody

Reactivity

Human

Target

Cathepsin B

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

SDRICIHTNAHVSVE

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.5-2 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

37.8

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 35kDa band observed in lysates of cell line HepG2 and in Human Lung, Human Colo-rectal carcinoma and Human Brain Glio-Blastoma cancer lysates (calculated MW of 37.8xkDa according to NP_001899.1) . Recommended concentration: 0.5-2μg/ml. Primary incu. IHC: Paraffin embedded Human Prostate. Recommended concentration: 5μg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ vesicles of HeLa cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

Tested Applications

ELISA, FC, IF, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

1508

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