Goat anti-Cathepsin B Antibody
Goat polyclonal antibody to CTSB
Product Specifications
Product Name Alternative
Anti-CTSB antibody, anti-cathepsin B antibody, anti-APPS antibody, anti-CPSB antibody, anti-APP secretase antibody, anti-amyloid precursor protein secretase antibody, anti-cathepsin B1 antibody, anti-cysteine protease antibody
Reactivity
Human
Target
Cathepsin B
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
SDRICIHTNAHVSVE
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.5-2 μg/ml, IHC-P: 5 μg/ml
Molecular Weight
37.8
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 35kDa band observed in lysates of cell line HepG2 and in Human Lung, Human Colo-rectal carcinoma and Human Brain Glio-Blastoma cancer lysates (calculated MW of 37.8xkDa according to NP_001899.1) . Recommended concentration: 0.5-2μg/ml. Primary incu. IHC: Paraffin embedded Human Prostate. Recommended concentration: 5μg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ vesicles of HeLa cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:
Tested Applications
ELISA, FC, IF, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
1508
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