Goat anti-GATA1 Antibody
Goat polyclonal antibody to GATA1
Product Specifications
Product Name Alternative
GATA1; GATA binding protein 1 (globin transcription factor 1) ; GF1; NFE1; ERYF1
Reactivity
Human
Target
GATA1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
PTTSTTVVAPLSS
Field of Research
Cancer Biology; Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
P-ELISA: 1:32000, IF/ICC: 10 μg/ml, FACS: 10 ug/ml, WB: 1-3μg/ml
Molecular Weight
42.8
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 50kDa band observed in nuclear lysates of cell line K562 (calculated MW of 42.8kDa according to NP_002040.1) . The observed molecular weight corresponds to different antibodies from other sources and was successfully blocked by incubation with the i. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
Tested Applications
ELISA, FC, IF, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
2623
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