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DAPI (dihydrochloride) (solution)

DAPI (4',6-Diamidino-2-phenylindole) dihydrochloride (solution) is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells[1]. DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment[2] (Ex/Em = 356/451 nm) .

Product Specifications

CAS Number

[28718-90-3]

Product Name Alternative

4',6-Diamidino-2-phenylindole dihydrochloride (solution)

UNSPSC

12171500

Hazard Statement

H315-H317-H335

Target

DNA Stain; Sodium Channel

Type

Dye Reagents

Related Pathways

Cell Cycle/DNA Damage; Membrane Transporter/Ion Channel

Field of Research

Neurological Disease

Assay Protocol

https://www.medchemexpress.com/dapi-dihydrochloride-solution.html

Concentration

28.55 μM * 50 mL in DDH2O

Purity

99.20

Smiles

N=C(C1=CC2=C(C=C1)C=C(C3=CC=C(C(N)=N)C=C3)N2)N.[H]Cl.[H]Cl

Molecular Formula

C16H17Cl2N5

Molecular Weight

350.25

Precautions

P261-P264-P271-P272-P280-P302+P352-P304+P340-P362+P364-P403+P233-P405-P501

References & Citations

[1]Kapuscinski J. DAPI: a DNA-specific fluorescent probe[J]. Biotechnic & histochemistry, 1995, 70 (5) : 220-233.|[2]Kuduk SD, et al. Amidine derived inhibitors of acid-sensing ion channel-3 (ASIC3) . Bioorg Med Chem Lett. 2009 Aug 1;19 (15) :4059-63.

Shipping Conditions

Blue Ice

Storage Conditions

Solution, -20°C, protect from light, 2 years

Scientific Category

Dye Reagents

Clinical Information

No Development Reported

Citation 01

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