Goat anti-SCRIB / Scribble Antibody
Goat polyclonal antibody to SCRIB
Product Specifications
Product Name Alternative
SCRIB; scribbled homolog (Drosophila) ; CRIB1; SCRB1; SCRIB1; Vartul; scribble
Reactivity
Human
Target
SCRIB / Scribble
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
PEGPGKEKELPGQ
Field of Research
Neuroscience; Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
P-ELISA: 1:1000., IF: 10 μg/ml
Molecular Weight
178; 175
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 200-250kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS (calculated MW of 178kDa according to NP_874365.3) . This molecular weight is routinely observed by other sources. Recommended concentration: 1-3μg/ml. Primary incubation 1 hour . Notes: Immunofluorescence: Strong expression of the protein seen in the cell junctions of MCF7 cells. Recommended concentration: 10μg/ml.
Tested Applications
ELISA, IF, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
23513
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