CHAPS

CHAPS is a non-covalent reversible stabilizer of nucleosomes derived from cholic acid (HY-N0324) . CHAPS can stabilize the ultrastructure of nucleosomes, inhibit the spontaneous dissociation of nucleosomes at low concentrations, and maintain the integrity of the basic structural units of chromatin. CHAPS can promote the sliding of histone cores along the DNA chain and reduce sequence-specific binding. CHAPS can also be used as a zwitterionic detergent to dissolve membrane proteins, stabilize various protein-DNA complexes, and retain the biochemical activity of proteins in solution[1][2].

Product Specifications

CAS Number

[75621-03-3]

UNSPSC

12352211

Hazard Statement

H302, H315, H319, H335

Target

Biochemical Assay Reagents

Type

Reference compound

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/CHAPS.html

Concentration

10mM

Purity

99.91

Solubility

DMSO : 116.67 mg/mL (ultrasonic) |H2O : 66.67 mg/mL

Smiles

O[C@@H]1CC[C@]2(C)[C@@]3([H])C[C@H](O)[C@]4(C)[C@@H]([C@H](C)CCC(NCCC[N+](C)(C)CCCS([O-])(=O)=O)=O)CC[C@@]4([H])[C@]3([H])[C@H](O)C[C@@]([H])2C1

Molecular Formula

C32H58N2O7S

Molecular Weight

614.88

Precautions

H302, H315, H319, H335

References & Citations

[1]Kroflic A, et al. Thermodynamic characterization of 3-[ (3-cholamidopropyl) -dimethylammonium]-1-propanesulfonate (CHAPS) micellization using isothermal titration calorimetry: temperature, salt, and pH dependence. Langmuir. 2012 Jul 17;28 (28) :10363-71.|[2]Menshikova I, et al. Nucleosomes structure and dynamics: effect of CHAPS. Int J Biochem Mol Biol. 2011;2 (2) :129-137.