Anti-STAT1α Antibody

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. BPubMed:12764129, PubMed:12855578, PubMed:15322115, PubMed:19088846, PubMed:26479788, PubMed:28753426, PubMed:8156998, PubMed:9724754}.

Immunoblotting : use a1:1,000 dilution. <br><br>Immunoprecipitation: use 2-4 ug antibody per sample. <br><br>Positive control: Whole cell lysate from HeLa cells, Jurkat cells, or A431 cells.

STATS (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. STAT1 is activated by many different ligands including the interferon family, gp130 family, and receptpor tyrosine kinases. STAT1 has two forms, the 91 kD STAT1alpha and the 84 kD STAT1beta, which are encoded by the same gene with splicing variant.