ML216

ML216 (CID49852229) is a potent, selective inhibitor of DNA unwinding activity of BLM (Bloom's syndrome protein) with IC50 of 2.98 and 0.97 uM for full length BLM and BLM636-1298, respectively; weak inhibits WRN helicase (IC50=12.6 uM) and does not inhibit RecQ1, RecQ5 and UrvD (IC50>50 uM) ; disrupts BLM’s binding to DNA, rather than being an inhibitor of ATP binding by BLM, the inhibition of ssDNA-dependent ATPase activity with Ki of 1.76 uM, shows cell-based activity and can induce sister chromatid exchanges, enhances the toxicity of aphidicolin, and exerts antiproliferative activity in cells expressing BLM.