IOX 2

IOX2 is a selective inhibitor of the Hypoxia Inducible Factor (HIF) Prolyl-Hydroxylases (PHD) ; active in cells with IC50 value of 21 nM for PHD2/ELGN-1 and no inhibition at FIH (20uM) . (In Vitro) :IOX2 (0, 10, 25, and 50 μM) dose-dependently inhibits collagen-related peptide (CRP; 0.25 μg/mL) or thrombin (0.03 U/mL) -induced platelet aggregation and ATP release. But IOX2 doesn’t affect P-selectin expression and the surface levels of glycoprotein (GP) Ibα, GPVI, or αIIbβ3.IOX2 also inhibits the spreading of platelets on fibrinogen or collagen and clot retraction.IOX2 (50 μM; 24 h) increases the transcription level of VEGF-A and BNIP3 in Normal human epidermal keratinocytes (NHEK) and Normal human dermal fibroblasts (NHDF), when grown under normoxia and hypoxia condition. (In Vivo) :IOX2 (10 mg/kg; i.p.; single dose) impaired the in vivo hemostatic function of platelets and arterial thrombus formation in mice.