RAT ANTI HUMAN CD3

It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.<br>Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Rat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3&epsilon; chain recognizes human CD3&epsilon;. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (&epsilon;, &gamma;, &delta;, &zeta;) that assemble and function as three pairs of dimers (&epsilon;&gamma;, &epsilon;&delta;, &zeta;&zeta;). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005).

[T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e molecule, epsilon (CD3-TCR complex); T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e]