2',4'-DIHYDROXYCHALCONE

2',4'-Dihydroxychalcone as a compound with Hsp90 inhibitory and antifungal effectsIn the presence of 2',4'-DHC (8 g/mL), radial growth of Aspergillus fumigatus was inhibited 20% compared to the control, and green pigmentation was completely blocked. The expression of the conidiation-associated genes abaA, brlA, and wetA was significantly decreased (approximately 3- to 5-fold) by treatment with 2',4'-DHC. The expression of calcineurin signaling components, cnaA and crzA, was also significantly reduced. The inhibitory effects of 2',4'-DHC on metabolic activity and mycelial growth were significantly enhanced by combination treatment with itraconazole and caspofungin. Docking studies indicated that 2',4'-DHC bind to the ATPase domain of Hsp90. 2',4'-DHC act as an Hsp90-calcinurin pathway inhibitor.