POU4F2 Antibody
Goat polyclonal antibody to POU4F2
Product Specifications
Product Name Alternative
Anti BRN3.2 antibody, anti BRN3B antibody, anti Brn-3b antibody, anti Brn3b POU domain transcription factor antibody, anti POU class 4 homeobox 2 antibody, anti POU domain class 4 transcription factor 2 antibody, anti POU domain protein antibody, anti POU domain, class 4, transcription factor 2 antibody, anti POU4F2 antibody
Reactivity
Human, Mouse, Rat
Target
POU4F2
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
HGLPSHMGCMSDVD
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.03 μg/ml
Molecular Weight
43.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Preliminary experiments gave an approx 70kDa band in Rat Retina lysates after 0.03μg/ml antibody staining.Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.2kDa according to NP_599182.1.The 70kDa band was successfully blocked by incubation with the immunizing peptide.We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates.Have any further splice variants/modified forms been reported.Experiment Notes: This antibody is not expected to cross-react to the similar POU4F3 (GeneID 5459)
Prediction Reactivity
Bovine, Canine
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
5458
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