EAAT1/GLAST1 (aa197-208) Antibody
Goat polyclonal antibody to SLC1A3
Product Specifications
Product Name Alternative
Anti SLC1A3 antibody, anti solute carrier family 1 (glial high affinity glutamate transporter), member 3 antibody, anti EA6 antibody, anti EAAT1 antibody, anti FLJ25094 antibody, anti GLAST antibody, anti GLAST1 antibody, anti GLAST-1 antibody, anti OTTHUMP00000116002 antibody, anti excitatory amino acid transporter 1 antibody, anti sodium-dependent glutamate/aspartate transporter 1 antibody, anti solute carrier family 1 member 3 antibody
Reactivity
Human
Target
EAAT1 / GLAST1 (aa197-208)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
RSFKVPIQANET
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.1-0.3 μg/ml
Molecular Weight
59.6; 55.1; 7.42
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 60kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 59.6kDa according to NP_004163.3) .Recommended concentration:0.1-0.3μg/ml. An additional band of unknown identity was also consistently observed at 22kDa.This band was successfully blocked by incubation with the immunizing peptide.We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates.Have any further splice variants/modified forms been reported.Experiment Notes: This antibody is expected to recognize isoform 1 (NP_004163.3) and isoform 2 (NP_001160167.1) .
Prediction Reactivity
Rat
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
6507
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