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Nitro blue tetrazolium chloride

Nitro blue tetrazolium chloride is a NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 M) . A well-known scavenger of superoxide anions.Nitro blue tetrazolium chloride, a substrate for dehydrogenases, is used with the alkaline phosphatase substrate BCIP in western blotting and immunohistological staining procedures.

Product Specifications

CAS Number

298-83-9

Product Name Alternative

NBT

Purity

>98% (HPLC)

Solubility

70%DMF:100 mg/mL (122.30 mM) ; DMSO:33.33 mg/mL (40.76 mM; Need ultrasonic)

Smiles

COC1=CC (C2=CC=C (N3N=C (C4=CC=CC=C4) N=[N+]3C5=CC=C ([N+] ([O-]) =O) C=C5) C (OC) =C2) =CC=C1N6N=C (C7=CC=CC=C7) N=[N+]6C8=CC=C ([N+] ([O-]) =O) C=C8.[Cl-].[Cl-]

Molecular Formula

C40H30Cl2N10O6

Molecular Weight

817.64

Storage Conditions

Storage temperature: -20°C. Stability: ≥ 2 years

Notes

For research use only.

Available Sizes

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