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TNNT1 Antibody

Goat polyclonal antibody to TNNT1

Product Specifications

Product Name Alternative

Anti TNNT1 antibody, anti troponin T type 1 (skeletal, slow) antibody, anti ANM antibody, anti FLJ98147 antibody, anti MGC104241 antibody, anti STNT antibody, anti TNT antibody, anti TNTS antibody, anti slow skeletal muscle troponin T antibody, anti troponin T, slow skeletal muscle antibody, anti troponin-T1, skeletal, slow antibody

Reactivity

Human

Target

TNNT1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

EKAQELSDWIHQLE

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

32.9; 31.2; 30.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Preliminary experiments gave an approx 50kDa band in Human Skeletal Muscle lysates after 0.1μg/ml antibody staining.Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.9kDa according to NP_003274.3.The 50kDa band was successfully blocked by incubation with the immunizing peptide.We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates.Have any further splice variants/modified forms been reported.Experiment Notes: This antibody is expected to recognize all reported isoforms (NP_003274.3, NP_001119604.1, NP_001119605.1) .

Prediction Reactivity

Bovine, Canine, Porcine

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

7138

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