Sirtuin 1/SIRT1 (aa577-590) Antibody
Goat Polyclonal to SIRT1
Product Specifications
Product Name Alternative
Anti sirtuin type 1 antibody, sir2-like 1 antibody, hSIR2 antibody, SIR2alpha antibody, SIR2-like protein 1 antibody, OTTHUMP00000198112 antibody, OTTHUMP00000198111 antibody, NAD-dependent deacetylase sirtuin-1 antibody, SIR2L1 antibody, sirtuin 1 antibody, SIRT1 antibody
Reactivity
Human
Target
Sirtuin 1 / SIRT1 (aa577-590)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
EKPQEVQTSRNVES
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.05-0.2 μg/ml, IHC-P: 3-5μg/ml
Molecular Weight
81.7; 50.5
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 120kDa band observed in lysates of cell line Jurkat (calculated MW of 81.7kDa according to NP_036370.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16;100 (19) :10794-9.; PMID: 12960381) . Recommended concentration: 0.05-0.2 μg/ml.
Prediction Reactivity
Mouse
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
23411
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