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Ac4GlcNAz

The tetraacetylated N-Azidoacetyl-glucosamine (Ac 4GlcNAz) provides a non-radioactive alternative for glycoconjugate vi-sualization. It is cell-permeable, intracellularly processed and incorporated instead of its natural monosaccharide counterpart N-Acetylglucosamine (GlcNAc) . The resulting Azide-functionalized glycoconjugates can subse-quently be detected via Cu (I) -catalyzed or Cu (I) -free Click Chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or to introduce fluorescent group (via Azides of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging. Recommended concentration for metabolic labeling: 25-75 M. This concentration range may serve as a starting point for an individual assay set-up.

Product Specifications

CAS Number

98924-81-3

Product Name Alternative

N-azidoacetylglucosamine-tetraacylated (Ac4GlcNAz)

Purity

Mass identification (ESI-MS)

Form

Amorphous solid; Color: off-white to grey

Solubility

DMSO, DMF, MeOH

Smiles

[C@H]1 ([C@H] (C (O[C@@H] ([C@H]1OC (=O) C) COC (=O) C) OC (=O) C) NC (=O) CN=[N+]=[N-]) OC (=O) C

Molecular Formula

C16H22N4O10

Molecular Weight

Theoretical MW: 430.37 g/mol; Detected MW: 430.13 g/mol

Storage Conditions

Store at -20 °C

Notes

For research use only.

Applications Notes

Glycoconjugate synthesis monitoring by metabolic labeling.

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