5-Ethynyl-dUTP (5-EdUTP)

Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Ventexo-or KOD XL) . Incorporation into DNA by Primer Extension with family A polymerase Taqand family B polymerases (Pwo, Deep Vent exo-or KOD XL) . The resulting ethynyl-functionalized DNA can subsequently be processed via Cu (I) -catalyzed click chemistry that offers the choice: to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks; to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins. Spectroscopic Propertie: max 288 nm, 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .