5-Ethynyl-UTP (5-EUTP)
5-Ethynyl-UTP (5-EUTP)
Product Specifications
Product Name Alternative
5-Ethynyl-uridine-5'-triphosphate, Sodium salt
Concentration
100 mM-110 mM
Purity
95% (HPLC)
Form
Solution in water; Color: colorless to slightly yellow; pH: 7.5 0.5
Smiles
N1 ([C@@H]2O[C@H] (COP (=O) (OP (=O) (OP (=O) (O) O) O) O) [C@H] ([C@H]2O) O) c (=O) [nH]c (=O) c (c1) C#C
Molecular Formula
C11H15N2O15P3
Molecular Weight
Theoretical MW: 508.16 g/mol (free acid) ; Detected MW: 507.97 g/mol (free acid)
Storage Conditions
Store at -20 C. Short-term exposure (up to 1 week cumulative) to ambient temperature possible.
Notes
For research use only.
Applications Notes
Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription. The resulting alkyne-functionalized RNA can subsequently be processed via Cu (I) -catalyzed (azide-alkyne) click chemistry that offers the choice: to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin) ; to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes) ; to crosslink the RNA to azide-functionalized biomolecules e.g.proteins. Spectroscopic Propertie: max 288 nm, 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
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