DdATP
DdATP (2',3'-Dideoxyadenosine-5'-triphosphate, Lithium salt) is a high-purity chain-terminating nucleotide analog widely used in DNA sequencing, primer extension, and molecular biology research. As a modified form of dATP, ddATP is enzymatically incorporated into DNA strands but lacks the essential 3'-OH group, resulting in immediate termination of DNA synthesis. This property makes it an essential reagent for Sanger sequencing and controlled DNA polymerization studies.
Product Specifications
CAS Number
24027-80-3
Product Name Alternative
2',3'-Dideoxyadenosine-5'-triphosphate, Lithium salt
Concentration
10 mM-11 mM
Purity
≥ 98% (HPLC)
Form
Solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Smiles
OP (=O) (O) OP (=O) (O) OP (=O) (O) OC[C@H]1O[C@@H] (n2cnc3c (N) ncnc23) CC1
Molecular Formula
C10H16N5O11P3
Molecular Weight
Theoretical MW: 475.18 g/mol (free acid) ; Detected MW: 475.01 g/mol (free acid)
Storage Conditions
Store at -20°C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Notes
For research use only.
Applications Notes
Spectroscopic Propertie: λmax 259 nm, ε 15.1 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
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