DdNTP Bundle
DdNTP Bundle contains four separate solutions of ddATP, ddCTP, ddGTP, and ddTTP at a concentration of 10-11 mM supplied as clear aqueous solutions (pH 7.5 ± 0.5) .
Product Specifications
Product Name Alternative
DdATP, ddCTP, ddGTP and ddTTP 10 mM solutions, 2',3'-Dideoxyadenosine-5'-triphosphate, Lithium salt; 2',3'-Dideoxycytidine-5'-triphosphate, Lithium salt; 2',3'-Dideoxyguanosine-5'-triphosphate, Lithium salt; 2',3'-Dideoxythymidine-5'-triphosphate, Lithium salt
Concentration
10 mM-11 mM
Purity
≥ 98% (HPLC)
Form
Solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Smiles
Nc1c2c (n (cn2) [C@@H]2O[C@H] (COP (=O) (O) OP (=O) (O) OP (=O) (O) O) CC2) ncn1.OP (=O) (O) OP (=O) (O) OP (=O) (O) OC[C@H]1O[C@H] (CC1) n1c2nc ([nH]c (=O) c2nc1) N.OP (=O) (O) OP (=O) (O) OP (=O) (O) OC[C@H]1O[C@H] (CC1) n1ccc (nc1=O) N.OP (=O) (O) OP (=O) (O) OP (=O) (O) OC[C@H]1O[C@H] (CC1) n1c (=O) [nH]c (=O) c (c1) C
Molecular Formula
DdATP: C 10H16N5O11P3; ddCTP: C 9H16N3O12P3; ddGTP: C 10H16N5O12P3; ddTTP: C 10H17N2O13P3
Molecular Weight
Theoretical MW: ddATP: 475.18 g/mol (free acid), ddCTP: 451.16 g/mol (free acid), ddGTP: 491.18 g/mol (free acid), ddTTP: 466.17 g/mol (free acid)
Storage Conditions
Store at -20 °C, Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Notes
For research use only.
Applications Notes
Spectroscopic Propertie: ddATP: λmax 259 nm, ε 15.1 L mmol-1cm-1 (Tris-HCl pH 7.5), ddCTP: λmax 271 nm, ε 8.9 L mmol-1cm-1 (Tris-HCl pH 7.5), ddGTP: λmax 252 nm, ε 14.2 L mmol-1cm-1 (Tris-HCl pH 7.5), ddTTP: λmax 267 nm, ε 9.6 L mmol-1cm-1 (Tris-HCl pH 7.5) .
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