5-Methyl-dCTP
5-methylated DNA probes can be used as methylation reference fragment or for pull-down of 5-hmC binding proteins from cellular lysate.
Product Specifications
CAS Number
22003-12-9
Product Name Alternative
5-mdCTP, 5-Methyl-2'-deoxycytidine-5'-triphosphate, Sodium salt
Concentration
100 mM-110 mM
Purity
≥ 95% (HPLC)
Form
Solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Smiles
Cc1cn (c (=O) nc1N) [C@@H]1O[C@H] (COP (=O) (O) OP (=O) (O) OP (=O) (O) O) [C@@H] (O) C1
Molecular Formula
C10H18N3O13P3
Molecular Weight
Theoretical MW: 481.18 g/mol (free acid) ; Detected MW: 481.01 g/mol (free acid)
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Incorporation into DNA by-PCR with Taqpolymerasein-house data. Spectroscopic Propertie: λmax 277 nm, ε 9.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
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