5-Azido-C3-UTP

Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription (up to 1400 bp tested) . For a comprehensive protocol please refer to PP-501 or PP-501-Cy3/Cy5. 3'-Azide-Labeling of T7 promotor-containing oligonucleotides. The resulting azide-functionalized RNA can subsequently be processed via Cu (I) -free (azide-DBCO) or Cu (I) -catalyzed (azide-alkyne) click chemistry that offers the choice: to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively) ; to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively) ; to crosslink the RNA to azide-or alkyne functionalized biomolecules e.g.proteins. Spectroscopic Propertie: λmax 260 nm, ε 8.8 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .