5-Azido-C3-UTP
5-Azido-C3-UTP
Product Specifications
Product Name Alternative
5- (3-Azidopropyl) -uridine-5'-triphosphate, Triethylammonium salt
Concentration
10 mM-11 mM
Purity
≥ 95% (HPLC)
Form
Solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Smiles
C (CCc1cn (c (=O) [nH]c1=O) [C@H]1[C@@H] ([C@@H] ([C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O) O) O) N=[N+]=[N-]
Molecular Formula
C12H20N5O15P3
Molecular Weight
Theoretical MW: 567.23 g/mol (free acid) ; Detected MW: 567.02 g/mol (free acid)
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription (up to 1400 bp tested) . For a comprehensive protocol please refer to PP-501 or PP-501-Cy3/Cy5. 3'-Azide-Labeling of T7 promotor-containing oligonucleotides. The resulting azide-functionalized RNA can subsequently be processed via Cu (I) -free (azide-DBCO) or Cu (I) -catalyzed (azide-alkyne) click chemistry that offers the choice: to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively) ; to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively) ; to crosslink the RNA to azide-or alkyne functionalized biomolecules e.g.proteins. Spectroscopic Propertie: λmax 260 nm, ε 8.8 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
