Azide-PEG4-aminoallyl-dUTP
Azide-PEG 4-aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing Azide-functionalized DNA/cDNA can subsequently be labeled via Cu (I) -catalyzed or Cu (I) -free click chemistry that offers the choice: to introduce a Biotin group (via Alkynes of Biotin or DBCO-functionalized Biotin, respectively) for subsequent purification tasks; to introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectively) for sub-sequent microscopic imaging; to crosslink the DNA to Azide-or DBCO-functionalized biomolecules e.g.proteins.
Product Specifications
Product Name Alternative
5- (15-Azido-4,7,10,13-tetraoxa-pentadecanoyl-aminoallyl) -2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
Concentration
10 mM-11 mM
Purity
≥ 95% (HPLC)
Form
Solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Smiles
[C@@H]1 (C[C@@H] (O[C@@H]1COP (=O) (OP (=O) (OP (=O) (O) O) O) O) n1c (=O) [nH]c (=O) c (c1) /C=C/CNC (=O) CCOCCOCCOCCOCCN=[N+]=[N-]) O
Molecular Formula
C23H39N6O19P3
Molecular Weight
Theoretical MW: 796.51 g/mol (free acid) ; Detected MW: 796.15 g/mol (free acid)
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Frequently Asked Questions
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