What is the catalog number for Azide-PEG4-aminoallyl-dUTP?

The catalog number for Azide-PEG4-aminoallyl-dUTP is orb65282.

What sizes are available for Azide-PEG4-aminoallyl-dUTP?

Azide-PEG4-aminoallyl-dUTP is available in the following size(s): 5 x 50 ul.

Is Azide-PEG4-aminoallyl-dUTP in stock?

Azide-PEG4-aminoallyl-dUTP is currently made to order / not held in immediate stock. Contact us for lead time.

Azide-PEG4-aminoallyl-dUTP

Azide-PEG 4-aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing Azide-functionalized DNA/cDNA can subsequently be labeled via Cu (I) -catalyzed or Cu (I) -free click chemistry that offers the choice: to introduce a Biotin group (via Alkynes of Biotin or DBCO-functionalized Biotin, respectively) for subsequent purification tasks; to introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectively) for sub-sequent microscopic imaging; to crosslink the DNA to Azide-or DBCO-functionalized biomolecules e.g.proteins.

Product Specifications

Product Name Alternative

5- (15-Azido-4,7,10,13-tetraoxa-pentadecanoyl-aminoallyl) -2'-deoxyuridine-5'-triphosphate, Triethylammonium salt

Concentration

10 mM-11 mM

Purity

≥ 95% (HPLC)

Form

Solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5

Smiles

[C@@H]1 (C[C@@H] (O[C@@H]1COP (=O) (OP (=O) (OP (=O) (O) O) O) O) n1c (=O) [nH]c (=O) c (c1) /C=C/CNC (=O) CCOCCOCCOCCOCCN=[N+]=[N-]) O

Molecular Formula

C23H39N6O19P3

Molecular Weight

Theoretical MW: 796.51 g/mol (free acid) ; Detected MW: 796.15 g/mol (free acid)

Storage Conditions

Store at -20 °C

Notes

For research use only.

Applications Notes

Incorporation into DNA/cDNA by PCR with Taqpolymerasein-house data; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) . Spectroscopic Propertie: λmax 289 nm, ε 7.1 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .