Biotin-16-ddUTP

Biotin-16- (5-aminoallyl) -ddUTP

Product Specifications

CAS Number

188755-42-2 (Tetralithium salt), 748750-05-2 (free acid)

Product Name Alternative

Biotin-16- (5-aminoallyl) -ddUTP, Biotinyl-ε-aminocaproyl-γ-aminobutyryl-5- (3-aminoallyl) -2',3'-dideoxyuridine-5'-triphosphate, Triethylammonium salt

Concentration

1.0 mM-1.1 mM

Purity

≥ 95% (HPLC)

Form

Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5

Smiles

C1[C@H]2[C@@H] ([C@@H] (S1) CCCCC (=O) NCCCCCC (=O) NCCCC (=O) NC/C=C/c1c (=O) [nH]c (=O) n ([C@H]3CC[C@H] (O3) COP (=O) (OP (=O) (OP (=O) (O) O) O) O) c1) NC (=O) N2

Molecular Formula

C32H52N7O17P3S

Molecular Weight

Theoretical MW: 931.78 g/mol (free acid) ; Detected MW: 931.24 g/mol (free acid)

Storage Conditions

Store at -20 °C

Notes

For research use only.

Applications Notes

Technique for nanoscale patterning of functional molecules on the surface of a DNA origami; 3'-end labeling of oligonucleotides with Terminal Transferase, recombinant and labeling DNA; MALDI-TOF MS-based SNP typing assay; Biotinylation of in vitro transcribed tmRNA during surface plas-mon resonance (SPR) technique; Use in the standard nick translation reaction for tumor DNA la-belling during comparative genomic hybridization. Spectroscopic Propertie: λmax 240/289 nm, ε 10.7/7.1 L mmol-1 cm-1, (Tris-HCl pH 7.5) .