Biotin-11-dUTP
Biotin-11-dUTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended Biotin-11-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-11-dUTP/50% dTTP.
Product Specifications
Product Name Alternative
Biotin-X- (5-aminoallyl) -dUTP, γ-[N- (Biotin-6-amino-hexanoyl) ]- (5-aminoallyl) -2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Smiles
Molecular Formula
C28H45N6O17P3S
Molecular Weight
Theoretical MW: 862.67 g/mol (free acid) ; Detected MW: 862.18 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Notes
For research use only.
Applications Notes
Frequently Asked Questions
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