Aminoallyl-dUTP-Cy5
Aminoallyl-dUTP-Cy5 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-Cy5/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-Cy5/70-50% dTTP.
Product Specifications
Product Name Alternative
5- (3-Aminoallyl) -2'-deoxyuridine-5'-triphosphate, labeled with Cy5, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: blue; pH: 7.5 ± 0.5
Smiles
Molecular Formula
C45H58N5O21P3S2
Molecular Weight
Theoretical MW: 1162.01 g/mol (free acid) ; Detected MW: 1161.23 g/mol (free acid)
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Frequently Asked Questions
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