What is the catalog number for Aminoallyl-dUTP-Texas Red?

The catalog number for Aminoallyl-dUTP-Texas Red is orb64128.

What sizes are available for Aminoallyl-dUTP-Texas Red?

Aminoallyl-dUTP-Texas Red is available in the following size(s): 5 x 10 ul.

Is Aminoallyl-dUTP-Texas Red in stock?

Aminoallyl-dUTP-Texas Red is currently made to order / not held in immediate stock. Contact us for lead time.

Aminoallyl-dUTP-Texas Red

Aminoallyl-dUTP-Texas Red is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-Texas Red/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-Texas Red/80-70% dTTP (PCR), 50% Aminoallyl-dUTP-Texas Red/50% dTTP (Nick Translation) .

Product Specifications

Product Name Alternative

TexasRed-12-dUTP, 5- (3-Aminoallyl) -2'-deoxyuridine-5'-triphosphate, labeled with Texas Red, Triethylammonium salt

Concentration

1.0 mM-1.1 mM

Purity

≥ 95% (HPLC)

Form

Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: red-violet; pH: 7.5 ± 0.5

Smiles

P (=O) (OP (=O) (O) O) (O) OP (=O) (O) OC[C@H]1O[C@H] (C[C@@H]1O) n1c (=O) [nH]c (=O) c (c1) /C=C/CNC (=O) CCCCCNS (=O) (=O) c1cc (c (cc1) C1=c2c (Oc3c1cc1c4c3CCCN4CCC1) c1c3=[N+] (CCCc3c2) CCC1) S (=O) (=O) [O-].P (=O) (OP (=O) (O) O) (O) OP (=O) (O) OC[C@H]1O[C@H] (C[C@@H]1O) n1c (=O) [nH]c (=O) c (c1) /C=C/CNC (=O) CCCCCNS (=O) (=O) c1cc (ccc1C1=c2c (Oc3c1cc1c4c3CCCN4CCC1) c1c3=[N+] (CCCc3c2) CCC1) S (=O) (=O) [O-]

Molecular Formula

C49H59N6O21P3S2

Molecular Weight

Theoretical MW: 1225.07 g/mol (free acid) ; Detected MW: 1224.24 g/mol (free acid)

Storage Conditions

Store at -20 °C

Notes

For research use only.

Applications Notes

Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 588 nm, λem 609 nm, ε 80.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) .