5-Propargylamino-dCTP-ATTO-647N
5-Propargylamino-dCTP-ATTO-647N is recommended for direct enzy-matic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-ATTO-647N/dCTP ratio for Nick Translation: 30-50% Propargylamino-dCTP-ATTO-647N/70-50% dCTP.
Product Specifications
Product Name Alternative
5-Propargylamino-2'-deoxycytidine-5'-triphosphate, labeled with ATTO 647N, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Solution in water; Color: blue; pH: 7.5 ± 0.5
Smiles
Molecular Formula
C54H68N7O15P3
Molecular Weight
Theoretical MW: 1148.08 g/mol (free acid) ; Detected MW: 1147.40 g/mol (free acid)
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Spectroscopic Propertie: λexc 646 nm, λem 664 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) .
Frequently Asked Questions
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