5-Propargylamino-dCTP-Texas Red
5-Propargylamino-dCTP-Texas Red is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluo-rescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-TexasRed/dCTP ratio for PCR and Nick Translation: 20-30% Propargylamino-dCTP-TexasRed7/80-70% dCTP (PCR), 30-50% Propargylamino-dCTP-TexasRed/70-50% dCTP (Nick Translation) .
Product Specifications
Product Name Alternative
5-Propargylamino-2'-deoxycytidine-5'-triphosphate, labeled with Texas Red, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Solution in water; Color: red-violet; pH: 7.5 ± 0.5
Smiles
Molecular Formula
C49H58N7O20P3S2
Molecular Weight
Theoretical MW: 1222.07 g/mol (free acid) ; Detected MW: 1221.24 g/mol (free acid)
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Frequently Asked Questions
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