Biotin-14-dCTP
Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties for Nick Translation are ensured by a 14-atom linker attached to the N4 position of cytosine. For PCR incorporation experiments e.g. with Taqpolymerase Biotin-11-dCTP or Biotin-16-dCTP are recommended whose Biotin moiety is attached to C5 position of cytidine via a 11-atom or 16-atom linker, respectively. Recommended Biotin-14-dCTP/dCTP ratio for Nick Translation: 50% Biotin-14-dCTP/50% dCTP.
Product Specifications
Product Name Alternative
Bio-14-dCTP, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Smiles
OP (=O) (O) OP (=O) (O) OP (=O) (O) OC[C@H]1O[C@@H] (n2ccc (nc2=O) NCCCCCCNC (=O) CCCCCNC (=O) CCCC[C@@H]2SC[C@@H]3NC (=O) N[C@H]23) C[C@@H]1O
Molecular Formula
C31H54N7O16P3S
Molecular Weight
Theoretical MW: 905.78 g/mol (free acid) ; Detected MW: 905.26 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Notes
For research use only.
Applications Notes
Frequently Asked Questions
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