HighYield T7 Fluorescein RNA Labeling Kit (UTP-based)

HighYield T7 Fluorescein RNA Labeling Kit (UTP-based) is designed to produce randomly Fluorescein-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. The labeling principle is similar to the underlying labeling principles of Fluorescein RNA Labeling Mix (Roche) . Fluorescein-12-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35% Fluorescein-12-UTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual op-timization of Fluorescein-12-UTP/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Fluorescein-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 μl each (35% Fluorescein-12-UTP substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM Fluorescein-12-UTP) .